Flow Cytometry Specialist II

Job Summary

Under the supervision of the Director of Flow Cytometry, Flow Cytometry Specialist II is responsible for assisting in the daily operation of the Flow Cytometry Core Facility.  The Flow Cytometry Core Facility provides cell-sorting and analysis services to the whole institute, as well as Kyowa Hakko Kirin, and other La Jolla Institute for Immunology (LJI) collaborators.  The Flow Cytometry Specialist II supports facility staff with calibration, operation, and maintenance of all sorters and analyzers. The Flow Cytometry Specialist II is responsible for providing guidance and assistance to new and existing facility users. 

Essential Duties and Responsibilities

·      Works under supervision of Flow Cytometry Specialists (III, and/or IV) and Director of Flow Cytometry; must be able to work independently 

·      Provide assistance to other Flow Cytometry Specialists and Director of Flow Cytometry

·      All duties and responsibilities of Flow Cytometry Specialist I

·      Perform daily calibration, operation, shutdown, routine maintenance, troubleshooting, and basic repair of all sorters and analyzers 

·      Perfom low, medium, and high-speed bulk and custom multi-parameter cell sorting of various cell lines and cell types from murine and human tissue/organs

·      Weekly cleaning and organization of all analyzer rooms and main sorter room

·      Weekly decontamination of all sorters and monthly cleaning of all analyzers

·      Weekly testing for contamination of sort buffers and tanks under sterile tissue culture conditions

·      Weekly/monthly data archive and database management of all sorters and analyzers 

·      Update instrument configurations and re-define baseline on all sorters and analyzers

·      Assist facility users in multi-parameter data acquisition and analysis using various software including: Diva, CellQuest Pro, and FlowJo

·      Provide guidance and assistance to new and existing facility users regarding protocols, instrument operation and data analysis

Leveling Requirements 

Requires a high school diploma, AA degree, or equivalent experience with a scientific background.  BS in science degree preferred with 2-4 years relevant work experience.  Must be able to work flexible hours. 

How to Apply:

Interested applicants please submit the following in a single attachment:

1. A brief cover letter relating how your talents fulfill the criteria for this position 
2. Your resume/CV
3. A minimum of two references (three preferred)